SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2) (Center) antibody

Antigen: SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2)

Synonyms: SHANK, CORTBP1, CTTNBP1, ProSAP1, SPANK-3, KIAA1022, mKIAA1022, SHANK2, shank2, MGC115330, MGC122482, CortBP1, Spank-3

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656710

Additional Information

Alternative name: SHANK2

Gene ID: 22941

UniProt: NP_036441.2, Q9UPX8

Immunogen: This SHANK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 886-916 amino acids from the Central region of human SHANK2.

Isotype: Ig

Description: Other names: SH3 and multiple ankyrin repeat domains protein 2,SHANK2,CORTBP1, KIAA1022, PROSAP1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SHANK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 886-916 amino acids from the Central region of human SHANK2.

Molecular Weight: 158822 DA

Comments:

Background: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain, and a proline-rich region. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. Two alternative splice variants, encoding distinct isoforms, are reported. Additional splice variants exist but their full-length nature has not been determined. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: SHANK2 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Neurology

Restrictions: For Research Use only

Images

SHANK2 Antibody (Center) (ABIN656710) western blot analysis in mouse bladder tissue lysates (35ug/lane). This demonstrates the SHANK2 antibody detected the SHANK2 protein (arrow).

Publications

Product: Lee, Lee, Kim et al.: "BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 8104-13, 2010 (PubMed).

Berkel, Marshall, Weiss et al.: "Mutations in the SHANK2 synaptic scaffolding gene in autism spectrum disorder and mental retardation." in: Nature genetics, Vol. 42, Issue 6, pp. 489-91, 2010 (PubMed).

Pinto, Pagnamenta, Klei et al.: "Functional impact of global rare copy number variation in autism spectrum disorders." in: Nature, Vol. 466, Issue 7304, pp. 368-72, 2010 (PubMed).