CCR4-NOT Transcription Complex, Subunit 8 (CNOT8) (N-Term) antibody

Antigen: CCR4-NOT Transcription Complex, Subunit 8 (CNOT8)

Synonyms: CAF1, POP2, CALIF, hCAF1, AA536816, AU015770, AU043059, 1500015I04Rik, 1810022F04Rik, MGC94581, MGC52767, zgc:63844, wu:fe49a05, caf1, pop2, calif, hcaf1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656816

Additional Information

Alternative name: CNOT8

Gene ID: 9337

UniProt: NP_004770.4, Q9UFF9

Immunogen: This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human CNOT8.

Isotype: Ig

Description: Other names: CCR4-NOT transcription complex subunit 8,CNOT8,CALIF, POP2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human CNOT8.

Molecular Weight: 33540 DA

Comments:

Background: Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CNOT8 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CNOT8 (arrow) using rabbit polyclonal CNOT8 Antibody (N-term) (ABIN656816). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CNOT8 gene.

Publications

Product: Lau, Kolkman, van Schaik et al.: "Human Ccr4-Not complexes contain variable deadenylase subunits." in: The Biochemical journal, Vol. 422, Issue 3, pp. 443-53, 2009 (PubMed).

Aslam, Mittal, Koch et al.: "The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation." in: Molecular biology of the cell, Vol. 20, Issue 17, pp. 3840-50, 2009 (PubMed).

Piao, Zhang, Wu et al.: "CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells." in: Molecular and cellular biology, Vol. 30, Issue 6, pp. 1486-94, 2010 (PubMed).

Marchese, Aubareda, Tudor et al.: "MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27590-600, 2010 (PubMed).

Ruan, Osawa, Hosoda et al.: "Quantitative characterization of Tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27624-31, 2010 (PubMed).