Single-Minded Homolog 1 (Drosophila) (SIM1) (N-Term) antibody

Antigen: Single-Minded Homolog 1 (Drosophila) (SIM1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656884

Additional Information

Alternative name: SIM1

Gene ID: 6492

UniProt: P81133, NP_005059.2

Immunogen: This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1.

Isotype: Ig

Description: Other names: Single-minded homolog 1,SIM1,BHLHE14

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1.

Molecular Weight: 85515 DA

Comments:

Background: SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: SIM1 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Traurig, Mack, Hanson et al.: "Common variation in SIM1 is reproducibly associated with BMI in Pima Indians." in: Diabetes, Vol. 58, Issue 7, pp. 1682-9, 2009 (PubMed).