CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (S. Cerevisiae) (CHTF18) (C-Term) antibody

Antigen: CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (S. Cerevisiae) (CHTF18)

Synonyms: Chl12, MGC38008, 6030457M03Rik, CHTF18, MGC113153, MGC192547, zgc:113153, CHL12, Ctf18, RUVBL, C16orf41, C321D2.2, C321D2.3, C321D2.4, MGC81266

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656910

Additional Information

Alternative name: CHTF18

Gene ID: 63922

UniProt: Q8WVB6, NP_071375.1

Immunogen: This CHTF18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 913-943 amino acids from the C-terminal region of human CHTF18.

Isotype: Ig

Description: Other names: Chromosome transmission fidelity protein 18 homolog,CHTF18,C16orf41, CTF18

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CHTF18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 913-943 amino acids from the C-terminal region of human CHTF18.

Molecular Weight: 107383 DA

Comments:

Background: CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1, MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176], Bermudez et al., 2003 [PubMed 12930902]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CHTF18 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

CHTF18 Antibody (C-term) (ABIN656910) western blot analysis in NCI-H292 cell line lysates (35ug/lane). This demonstrates the CHTF18 antibody detected the CHTF18 protein (arrow).

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Shiomi, Masutani, Hanaoka et al.: "A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity." in: The Journal of biological chemistry, Vol. 282, Issue 29, pp. 20906-14, 2007 (PubMed).

Farina, Shin, Kim et al.: "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1." in: The Journal of biological chemistry, Vol. 283, Issue 30, pp. 20925-36, 2008 (PubMed).

Murakami, Takano, Takeo et al.: "Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-replication factor C (RFC) and DNA polymerase {epsilon} is mediated by the cohesion-specific subunits, Ctf18, Dcc1, and Ctf8." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34608-15, 2010 (PubMed).