alpha Ketoglutarate Dehydrogenase (alphaKGDHC) (C-Term) antibody

Antigen: alpha Ketoglutarate Dehydrogenase (alphaKGDHC)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657365

Additional Information

Alternative name: OGDH

Gene ID: 4967

UniProt: NP_002532.2, Q02218

Immunogen: This OGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the C-terminal region of human OGDH.

Isotype: Ig

Description: Other names: 2-oxoglutarate dehydrogenase, mitochondrial,OGDH,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This OGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the C-terminal region of human OGDH.

Molecular Weight: 115935 DA

Comments:

Background: This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: OGDH Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Signaling, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

OGDH Antibody (C-term) (ABIN657365) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the OGDH antibody detected the OGDH protein (arrow).

Publications

Product: McCartney, Rice, Sanderson et al.: "Subunit interactions in the mammalian alpha-ketoglutarate dehydrogenase complex. Evidence for direct association of the alpha-ketoglutarate dehydrogenase and dihydrolipoamide dehydrogenase components." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 24158-64, 1998 (PubMed).

Habelhah, Laine, Erdjument-Bromage et al.: "Regulation of 2-oxoglutarate (alpha-ketoglutarate) dehydrogenase stability by the RING finger ubiquitin ligase Siah." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53782-8, 2004 (PubMed).