Ral Guanine Nucleotide Dissociation Stimulator-Like 2 (RGL2) (N-Term) antibody

Antigen: Ral Guanine Nucleotide Dissociation Stimulator-Like 2 (RGL2)

Synonyms: KE1.5, RAB2L, HKE1.5, Rlf, Rgt2, Rab2l, rab2l, im:7136742, Ke1.5, MGC124925, RGL2, MGC139909

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657382

Additional Information

Alternative name: RGL2

Gene ID: 5863

UniProt: O15211, NP_004752.1

Immunogen: This RGL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human RGL2.

Isotype: Ig

Description: Other names: Ral guanine nucleotide dissociation stimulator-like 2,RGL2,RAB2L

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RGL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human RGL2.

Molecular Weight: 83549 DA

Comments:

Background: Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: RGL2 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

RGL2 Antibody (N-term) (ABIN657382) western blot analysis in uterine tumor cell line lysates (35ug/lane). This demonstrates the RGL2 antibody detected the RGL2 protein (arrow).

Publications

Product: Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Wu, Ma, Brown et al.: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).

Ferro, Magrini, Guazzi et al.: "G-protein binding features and regulation of the RalGDS family member, RGL2." in: The Biochemical journal, Vol. 415, Issue 1, pp. 145-54, 2008 (PubMed).

Barcellos, May, Ramsay et al.: "High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000696, 2009 (PubMed).

Vigil, Martin, Williams et al.: "Aberrant overexpression of the Rgl2 Ral small GTPase-specific guanine nucleotide exchange factor promotes pancreatic cancer growth through Ral-dependent and Ral-independent mechanisms." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34729-40, 2010 (PubMed).