Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (C-Term) antibody

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyms: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657457

Additional Information

Alternative name: FCGR3A

Gene ID: 2214

UniProt: P08637, NP_001121067.1, NP_001121064.1, NP_000560.5, NP_001121068.1, NP_001121065.1

Immunogen: This FCGR3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the C-terminal region of human FCGR3A.

Isotype: Ig

Description: Other names: Low affinity immunoglobulin gamma Fc region receptor III-A,FCGR3A,CD16A, FCG3, FCGR3, IGFR3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FCGR3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the C-terminal region of human FCGR3A.

Molecular Weight: 29089 DA

Comments:

Background: This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: FCGR3A Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

FCGR3A Antibody (C-term) (ABIN657457) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the FCGR3A antibody detected the FCGR3A protein (arrow).

Publications

Product: Dornan, Spleiss, Yeh et al.: "Effect of FCGR2A and FCGR3A variants on CLL outcome." in: Blood, Vol. 116, Issue 20, pp. 4212-22, 2010 (PubMed).

Li, Chen, Evens et al.: "Rituximab-induced late-onset neutropenia in newly diagnosed B-cell lymphoma correlates with Fc receptor FcγRIIIa 158(V/F) polymorphism." in: American journal of hematology, Vol. 85, Issue 10, pp. 810-2, 2010 (PubMed).