Helt BHLH Transcription Factor (HELT) (Center) antibody

Antigen: Helt BHLH Transcription Factor (HELT)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657547

Additional Information

Alternative name: HELT

Gene ID: 391723

UniProt: A6NFD8, NP_001025058.1

Immunogen: This HELT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the Central region of human HELT.

Isotype: Ig

Description: Other names: Hairy and enhancer of split-related protein HELT,HELT,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HELT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the Central region of human HELT.

Molecular Weight: 35816 DA

Comments:

Background: HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3' (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: HELT Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

HELT Antibody (Center) (ABIN657547) western blot analysis in mouse liver tissue lysates (35ug/lane). This demonstrates the HELT antibody detected the HELT protein (arrow).

Publications

Product: Nakatani, Mizuhara, Minaki et al.: "Helt, a novel basic-helix-loop-helix transcriptional repressor expressed in the developing central nervous system." in: The Journal of biological chemistry, Vol. 279, Issue 16, pp. 16356-67, 2004 (PubMed).

Katoh, Katoh: "Identification and characterization of human HESL, rat Hesl and rainbow trout hesl genes in silico." in: International journal of molecular medicine, Vol. 14, Issue 4, pp. 747-51, 2004 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).