Histone Cluster 2, H2aa3 (HIST2H2AA3) (N-Term) antibody

Antigen: Histone Cluster 2, H2aa3 (HIST2H2AA3)

Synonyms: H2A, H2A.2, H2A/O, H2A/q, H2AFO, H2a-615, HIST2H2AA, Hist2h2aa, Hist2h2aa1, Hist2h2aa3, H2A/R, HIST2H2AA3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657623

Additional Information

Alternative name: HIST2H2AA3

Gene ID: 8337, 723790

UniProt: NP_001035807.1, NP_003507.1, Q6FI13

Immunogen: This HIST2H2AA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST2H2AA3.

Isotype: Ig

Description: Other names: Histone H2A type 2-A,HIST2H2AA3,H2AFO, HIST2H2AA

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HIST2H2AA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST2H2AA3.

Molecular Weight: 14095 DA

Comments:

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the centromeric copy.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: HIST2H2AA3 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

HIST2H2AA3 Antibody (N-term) (ABIN657623) western blot analysis in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the HIST2H2AA3 antibody detected the HIST2H2AA3 protein (arrow).

Publications

Product: Hagiwara, Hidaka, Yamada: "Deimination of histone H2A and H4 at arginine 3 in HL-60 granulocytes." in: Biochemistry, Vol. 44, Issue 15, pp. 5827-34, 2005 (PubMed).

Bonenfant, Coulot, Towbin et al.: "Characterization of histone H2A and H2B variants and their post-translational modifications by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 3, pp. 541-52, 2006 (PubMed).

Cao, Tsukada, Zhang: "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing." in: Molecular cell, Vol. 20, Issue 6, pp. 845-54, 2005 (PubMed).

Boyne, Pesavento, Mizzen et al.: "Precise characterization of human histones in the H2A gene family by top down mass spectrometry." in: Journal of proteome research, Vol. 5, Issue 2, pp. 248-53, 2006 (PubMed).

Bergink, Salomons, Hoogstraten et al.: "DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A." in: Genes & development, Vol. 20, Issue 10, pp. 1343-52, 2006 (PubMed).