Keratin 6B (KRT6B) (Center) antibody

Antigen: Keratin 6B (KRT6B)

Synonyms: K6B, PC2, CK6B, KRTL1, mK6[b], Krt2-6b, BB144589, MGC141272, KRT6B, KRT6C, KRT6A

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657654

Additional Information

Alternative name: KRT6B

Gene ID: 3854

UniProt: P04259, NP_005546.2

Immunogen: This KRT6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the Central region of human KRT6B.

Isotype: Ig

Description: Other names: Keratin, type II cytoskeletal 6B,KRT6B,K6B, KRTL1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KRT6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the Central region of human KRT6B.

Molecular Weight: 60067 DA

Comments:

Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified, the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: KRT6B Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

KRT6B Antibody (Center) (ABIN657654) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the KRT6B antibody detected the KRT6B protein (arrow).

Publications

Product: Takahashi, Paladini, Coulombe: "Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms." in: The Journal of biological chemistry, Vol. 270, Issue 31, pp. 18581-92, 1995 (PubMed).