Keratin 6B antibody

Catalog No. ABIN7126618

Product Details anti-Keratin 6B Antibody

Target: Keratin 6B (KRT6B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6B antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Cross-Reactivity (Details): Human and Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT6B protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil or basal cell carcinoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Keratin 6B

Target: Keratin 6B (KRT6B)

Alternative Name: KRT6B (KRT6B Products)

Synonyms: CK-6B antibody, CK6B antibody, K6B antibody, KRTL1 antibody, PC2 antibody, KRT6C antibody, KRT6A antibody, KRT6B antibody, BB144589 antibody, Krt2-6b antibody, mK6[b] antibody, KERIV antibody, keratin 6B antibody, keratin, type II cytoskeletal 5 antibody, keratin, type II cytoskeletal 6B antibody, KRT6B antibody, Krt6b antibody, LOC100566088 antibody, LOC100593588 antibody

Background: CK6B, Keratin Type II Cytoskeletal 6B, KRT6B,Cytokeratin 6B (KRT6B) (Basal Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 56kDa

Gene ID: 3854, 700779

UniProt: P04259