GDP Dissociation Inhibitor 2 (GDI2) (Center) antibody

Antigen: GDP Dissociation Inhibitor 2 (GDI2)

Synonyms: Gdi3, fc09e02, zgc:55919, zgc:77120, wu:fc09e02, gdi2, MGC54003, GDI2, GDI-2, MGC84311

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657709

Additional Information

Alternative name: GDI2

Gene ID: 2665

UniProt: NP_001485.2, P50395, NP_001108628.1

Immunogen: This GDI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human GDI2.

Isotype: Ig

Description: Other names: Rab GDP dissociation inhibitor beta,GDI2,RABGDIB

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GDI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human GDI2.

Molecular Weight: 50663 DA

Comments:

Background: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: GDI2 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

GDI2 Antibody (Center) (ABIN657709) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the GDI2 antibody detected the GDI2 protein (arrow).

Publications

Product: Shin, Wang, Yim et al.: "Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7607-16, 2003 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Sun, Zhu, Wang et al.: "Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers." in: Biochimica et biophysica acta, Vol. 1774, Issue 6, pp. 764-71, 2007 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).