Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2) (N-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2)

Synonyms: UBCH8, FLJ25157, ube2e2, wu:fb38h07, ubch8

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657745

Additional Information

Alternative name: UBE2E2

Gene ID: 7325

UniProt: NP_689866.1, Q96LR5

Immunogen: This UBE2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human UBE2E2.

Isotype: Ig

Description: Other names: Ubiquitin-conjugating enzyme E2 E2,UBE2E2,UBCH8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human UBE2E2.

Molecular Weight: 22255 DA

Comments:

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: UBE2E2 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

UBE2E2 Antibody (N-term) (ABIN657745) western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane). This demonstrates the UBE2E2 antibody detected the UBE2E2 protein (arrow).

Publications

Product: Takeuchi, Iwahara, Saeki et al.: "Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme." in: Journal of biochemistry, Vol. 138, Issue 6, pp. 711-9, 2006 (PubMed).

Dubois, Trynka, Franke et al.: "Multiple common variants for celiac disease influencing immune gene expression." in: Nature genetics, Vol. 42, Issue 4, pp. 295-302, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Yamauchi, Hara, Maeda et al.: "A genome-wide association study in the Japanese population identifies susceptibility loci for type 2 diabetes at UBE2E2 and C2CD4A-C2CD4B." in: Nature genetics, Vol. 42, Issue 10, pp. 864-8, 2010 (PubMed).