Retinoic Acid Receptor, alpha (RARA) (C-Term) antibody

Antigen: Retinoic Acid Receptor, alpha (RARA)

Synonyms: RAR, NR1B1, Nr1b1, RARalpha1, RARA, rar-alpha

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657770

Additional Information

Alternative name: RARA

Gene ID: 5914

UniProt: P10276, NP_001138773.1, NP_000955.1, NP_001019980.1

Immunogen: This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-379 amino acids from the C-terminal region of human RARA.

Isotype: Ig

Description: Other names: Retinoic acid receptor alpha,RARA,NR1B1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-379 amino acids from the C-terminal region of human RARA.

Molecular Weight: 50771 DA

Comments:

Background: This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: RARA Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Confocal immunofluorescent analysis of RARA Antibody (C-term) (ABIN657770) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Publications

Product: Chen, Guidez, Rousselot et al.: "PLZF-RAR alpha fusion proteins generated from the variant t(11;17)(q23;q21) translocation in acute promyelocytic leukemia inhibit ligand-dependent transactivation of wild-type retinoic acid receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 3, pp. 1178-82, 1994 (PubMed).

Catalano, Dawson, Somana et al.: "The PRKAR1A gene is fused to RARA in a new variant acute promyelocytic leukemia." in: Blood, Vol. 110, Issue 12, pp. 4073-6, 2007 (PubMed).

Park, Kim, Um: "A novel cytoplasmic adaptor for retinoic acid receptor (RAR) and thyroid receptor functions as a Derepressor of RAR in the absence of retinoic acid." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 34269-78, 2010 (PubMed).

Asleson, Morgan, Smith et al.: "Amplification of the RARA gene in acute myeloid leukemia: significant finding or coincidental observation?" in: Cancer genetics and cytogenetics, Vol. 202, Issue 1, pp. 33-7, 2010 (PubMed).