tyrosine Kinase, Non-Receptor, 2 (TNK2) (Center, Tyr284) antibody

Antigen: tyrosine Kinase, Non-Receptor, 2 (TNK2)

Binding Site: Center, Tyr284

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657857

Additional Information

Alternative name: ACK1

Gene ID: 10188

UniProt: Q07912, NP_005772.3, NP_001010938.1

Immunogen: This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human ACK1.

Isotype: Ig

Description: Other names: Activated CDC42 kinase 1,10188,ACK1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human ACK1.

Molecular Weight: 114569 DA

Comments:

Background: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: ACK1 Antibody (Center Y284) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin, Signaling

Restrictions: For Research Use only

Images

ACK1 Antibody (Center Y284) (ABIN657857) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).

Publications

Product: Prieto-Echagüe, Gucwa, Craddock et al.: "Cancer-associated mutations activate the nonreceptor tyrosine kinase Ack1." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10605-15, 2010 (PubMed).

Chua, Lim, Tham et al.: "Somatic mutation in the ACK1 ubiquitin association domain enhances oncogenic signaling through EGFR regulation in renal cancer derived cells." in: Molecular oncology, Vol. 4, Issue 4, pp. 323-34, 2010 (PubMed).

Mahajan, Mahajan: "Shepherding AKT and androgen receptor by Ack1 tyrosine kinase." in: Journal of cellular physiology, Vol. 224, Issue 2, pp. 327-33, 2010 (PubMed).