Vesicle Transport through Interaction with T-SNAREs Homolog 1B (Yeast) (VTI1B) (Center) antibody

Antigen: Vesicle Transport through Interaction with T-SNAREs Homolog 1B (Yeast) (VTI1B)

Synonyms: VTI1, VTI2, VTI1L, VTI1-LIKE, GES30, SNARE, MVti1b, AU015348, Vti1-rp1, MGC63525, zgc:63525, VTI1B, MGC137210

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657873

Additional Information

Alternative name: VTI1B

Gene ID: 10490

UniProt: NP_006361.1, Q9UEU0

Immunogen: This VTI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human VTI1B.

Isotype: Ig

Description: Other names: Vesicle transport through interaction with t-SNAREs homolog 1B,10490,VTI1, VTI1L, VTI1L1, VTI2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VTI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human VTI1B.

Molecular Weight: 26688 DA

Comments:

Background: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: VTI1B Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

VTI1B Antibody (Center) (ABIN657873) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the VTI1B antibody detected the VTI1B protein (arrow).

Publications

Product: Martinez-Arca, Rudge, Vacca et al.: "A dual mechanism controlling the localization and function of exocytic v-SNAREs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 15, pp. 9011-6, 2003 (PubMed).

Yan, Sun, McNew et al.: "Ca2+ and N-ethylmaleimide-sensitive factor differentially regulate disassembly of SNARE complexes on early endosomes." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 18270-6, 2004 (PubMed).

Furuta, Fujita, Noda et al.: "Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 1001-10, 2010 (PubMed).