Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Storage
4 °C/-20 °C
Storage Comment
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bhat, Friedmann, Knörck, Hoxha, Leidinger, Backes, Meese, Keller, Rettig, Hoth, Qu, Schwarz: "Syntaxin 8 is required for efficient lytic granule trafficking in cytotoxic T lymphocytes." in: Biochimica et biophysica acta, Vol. 1863, Issue 7 Pt A, pp. 1653-64, (2016) (PubMed).
Golebiewska, Harper, Williams, Savage, Goggs, Fischer von Mollard, Poole: "Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability." in: The Journal of biological chemistry, Vol. 290, Issue 3, pp. 1536-45, (2015) (PubMed).
Ramirez, Khvotchev, Trauterman, Kavalali: "Vti1a identifies a vesicle pool that preferentially recycles at rest and maintains spontaneous neurotransmission." in: Neuron, Vol. 73, Issue 1, pp. 121-34, (2012) (PubMed).
Offenhäuser, Lei, Roy, Collins, Stow, Murray: "Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 6, pp. 762-73, (2011) (PubMed).
Qu, Pattu, Junker, Schwarz, Bhat, Kummerow, Marshall, Matti, Neumann, Pfreundschuh, Becherer, Rieger, Rettig, Hoth: "Docking of lytic granules at the immunological synapse in human CTL requires Vti1b-dependent pairing with CD3 endosomes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 12, pp. 6894-904, (2011) (PubMed).
Fraldi, Annunziata, Lombardi, Kaiser, Medina, Spampanato, Fedele, Polishchuk, Sorrentino, Simons, Ballabio: "Lysosomal fusion and SNARE function are impaired by cholesterol accumulation in lysosomal storage disorders." in: The EMBO journal, Vol. 29, Issue 21, pp. 3607-20, (2010) (PubMed).
Target
VTI1B
(Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))