Chymotrypsin-Like Elastase Family, Member 2A (CELA2A) (C-Term) antibody

Antigen: Chymotrypsin-Like Elastase Family, Member 2A (CELA2A)

Synonyms: Ela2, Ela-2, Ela2a, ELAII, Elaii1, PE-1, ELA2A, CELA2A, ela2, ela2a

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657926

Additional Information

Alternative name: CELA2A

Gene ID: 63036

UniProt: NP_254275.1, P08217

Immunogen: This CELA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human CELA2A.

Isotype: Ig

Description: Other names: Chymotrypsin-like elastase family member 2A,63036,ELA2A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CELA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human CELA2A.

Molecular Weight: 28888 DA

Comments:

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CELA2A Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

CELA2A Antibody (C-term) (ABIN657926) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the CELA2A antibody detected the CELA2A protein (arrow).

Publications

Product: Lausen, Liu, Fliegauf et al.: "ELA2 is regulated by hematopoietic transcription factors, but not repressed by AML1-ETO." in: Oncogene, Vol. 25, Issue 9, pp. 1349-57, 2006 (PubMed).

Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).