Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2) antibody

Antigen: Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2)

Synonyms: ALDM, ALDHI, ALDH-E2, MGC1806, aldh2, MGC63786, zgc:63786, MGC80785, aldm, aldhi, aldh-e2, MGC89020, ALDH2, MGC138997, rf2b, DKFZp469J1332

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658993

Additional Information

Alternative name: ALDH2

Gene ID: 217

UniProt: NP_000681

Immunogen: This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.

Isotype: IgG1, Ig

Description: Other names: ALDH-E2, ALDHI, ALDH class 2, Aldehyde dehydrogenase, mitochondrial,ALDM, ALDH2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.

Molecular Weight: 56381 DA

Comments:

Background: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~1000 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Stem Cells, Cell Structure

Restrictions: For Research Use only

Publications

Product: Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Zhou, Diao, Li et al.: "Association of genetic polymorphisms of aldehyde dehydrogenase-2 with esophageal squamous cell dysplasia." in: World journal of gastroenterology : WJG, Vol. 16, Issue 27, pp. 3445-9, 2010 (PubMed).

Hendershot, Otto, Collins et al.: "Evaluation of a brief web-based genetic feedback intervention for reducing alcohol-related health risks associated with ALDH2." in: Annals of behavioral medicine : a publication of the Society of Behavioral Medicine, Vol. 40, Issue 1, pp. 77-88, 2010 (PubMed).

Weng, Weng, Lu et al.: "Effects of alcohol-drinking behaviour and ADH1B and ALDH2 polymorphisms on basal DNA damage in human mononuclear cells as determined by the comet assay." in: Mutation research, Vol. 701, Issue 2, pp. 132-6, 2010 (PubMed).

Yang, Yokoyama, Yokoyama et al.: "Relationship between genetic polymorphisms of ALDH2 and ADH1B and esophageal cancer risk: a meta-analysis." in: World journal of gastroenterology : WJG, Vol. 16, Issue 33, pp. 4210-20, 2010 (PubMed).