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Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) antibody

Details for Product No. ABIN659002
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Antigen
Synonyms EZH2, enx-1, enx1, ezh1, kmt6, xez, ezh2, olezh2, ENX-1, ENX1, EZH1, EZH2b, KMT6, KMT6A, WVS, WVS2, Enx-1, Enx1h, mKIAA4065, eya2, im:7149338, zgc:136376, zgc:152758
Reactivity
Human
(111), (53), (42), (37), (37), (36), (25), (12), (1), (1)
Host
Mouse
(100), (15), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
(75), (35), (30), (30), (25), (15), (14), (7), (5), (2), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN659002
291.50 $
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Immunogen This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
Clone 144CT2-1-1-5
Isotype IgG1, Ig kappa
Specificity This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
Predicted Reactivity Monkey
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name EZH2
Background This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
Synonyms: Lysine N-methyltransferase 6, ENX-1, Enhancer of zeste homolog 2, Histone-lysine N-methyltransferase EZH2,KMT6, EZH2
Molecular Weight 85363 Da
Gene ID 2146
UniProt Q15910
Research Area Translation Factors
Application Notes IF = 1:10-50, WB = 1:100-500, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) antibody Fluorescent image of U251 cell stained with EZH2 Antibody.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).EZH2 immunoreactivity is localized to Nucleus significantly.
anti-Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) antibody (2) Western blot analysis of EZH2 antibody in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.
anti-Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) antibody (3) EZH2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Background publications Kunderfranco, Mello-Grand, Cangemi et al.: "ETS transcription factors control transcription of EZH2 and epigenetic silencing of the tumor suppressor gene Nkx3.1 in prostate cancer." in: PLoS ONE, Vol. 5, Issue 5, pp. e10547, 2010 (PubMed).

Wang, Ye, Yuan et al.: "EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis." in: World journal of gastroenterology : WJG, Vol. 16, Issue 19, pp. 2421-7, 2010 (PubMed).

Kikuchi, Kinoshita, Shimizu et al.: "Distinctive expression of the polycomb group proteins Bmi1 polycomb ring finger oncogene and enhancer of zeste homolog 2 in nonsmall cell lung cancers and their clinical and clinicopathologic significance." in: Cancer, Vol. 116, Issue 12, pp. 3015-24, 2010 (PubMed).

Toll, Dasgupta, Potoczek et al.: "Implications of enhancer of zeste homologue 2 expression in pancreatic ductal adenocarcinoma." in: Human pathology, Vol. 41, Issue 9, pp. 1205-9, 2010 (PubMed).

Tsai, Manor, Wan et al.: "Long noncoding RNA as modular scaffold of histone modification complexes." in: Science (New York, N.Y.), Vol. 329, Issue 5992, pp. 689-93, 2010 (PubMed).

Validation Images
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