Enhancer of Zeste Homolog 2 (Drosophila) (EZH1) antibody

Antigen: Enhancer of Zeste Homolog 2 (Drosophila) (EZH1)

Synonyms: eya2, MGC136376, im:7149338, zgc:136376, zgc:152758, MGC189404, EZH2, ezh2, KMT6, Enx-1, Enx1h, KIAA4065, MGC90723, mKIAA4065, KIAA0388, ENX1, EZH1, ENX-1, MGC9169, Ezh2, xez, ezh2-A, MGC79865, MGC136829, zgc:136829, olezh2

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659002

Additional Information

Alternative name: EZH2

Gene ID: 2146

UniProt: Q15910

Immunogen: This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.

Isotype: IgG1, IgK

Description: Other names: Lysine N-methyltransferase 6, ENX-1, Enhancer of zeste homolog 2, Histone-lysine N-methyltransferase EZH2,KMT6, EZH2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.

Molecular Weight: 85363 DA

Comments:

Background: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.

Synonyms: eya2, MGC136376, im:7149338, zgc:136376, zgc:152758, MGC189404, EZH2, ezh2, KMT6, Enx-1, Enx1h, KIAA4065, MGC90723, mKIAA4065, KIAA0388, ENX1, EZH1, ENX-1, MGC9169, Ezh2, xez, ezh2-A, MGC79865, MGC136829, zgc:136829, olezh2

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50-500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors

Restrictions: For Research Use only

Publications

Product: Kunderfranco, Mello-Grand, Cangemi et al.: "ETS transcription factors control transcription of EZH2 and epigenetic silencing of the tumor suppressor gene Nkx3.1 in prostate cancer." in: PLoS ONE, Vol. 5, Issue 5, pp. e10547, 2010 (PubMed).

Wang, Ye, Yuan et al.: "EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis." in: World journal of gastroenterology : WJG, Vol. 16, Issue 19, pp. 2421-7, 2010 (PubMed).

Kikuchi, Kinoshita, Shimizu et al.: "Distinctive expression of the polycomb group proteins Bmi1 polycomb ring finger oncogene and enhancer of zeste homolog 2 in nonsmall cell lung cancers and their clinical and clinicopathologic significance." in: Cancer, Vol. 116, Issue 12, pp. 3015-24, 2010 (PubMed).

Toll, Dasgupta, Potoczek et al.: "Implications of enhancer of zeste homologue 2 expression in pancreatic ductal adenocarcinoma." in: Human pathology, Vol. 41, Issue 9, pp. 1205-9, 2010 (PubMed).

Tsai, Manor, Wan et al.: "Long noncoding RNA as modular scaffold of histone modification complexes." in: Science (New York, N.Y.), Vol. 329, Issue 5992, pp. 689-93, 2010 (PubMed).