Autophagy Related 4D, Cysteine Peptidase (ATG4D) antibody

Antigen: Autophagy Related 4D, Cysteine Peptidase (ATG4D)

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659112

Additional Information

Alternative name: ATG4D

Gene ID: 84971

UniProt: NP_116274.3, Q86TL0

Immunogen: This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.

Isotype: IgG1 kappa

Description: Other names: Cysteine protease ATG4D,ATG4D,APG4D, AUTL4

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.

Molecular Weight: 52922 DA

Comments:

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~ 1:50~1000

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mariño, Uría, Puente et al.: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).

Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Betin, Lane: "Caspase cleavage of Atg4D stimulates GABARAP-L1 processing and triggers mitochondrial targeting and apoptosis." in: Journal of cell science, Vol. 122, Issue Pt 14, pp. 2554-66, 2009 (PubMed).