ATG4D antibody (N-Term)

Catalog No. ABIN1882161

Cited in 7+ publications. The Rabbit Polyclonal anti-ATG4D antibody (Clone RB7563) (ABIN1882161) specifically detects ATG4D in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for ATG4D antibody (N-Term) (ABIN1882161)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7563

Product Details anti-ATG4D Antibody

Binding Specificity: AA 14-43, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human ATG4D.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATG4D antibody (ABIN1882161)

Williamson, Zhang, Colberg-Poley: "The human cytomegalovirus protein UL37 exon 1 associates with internal lipid rafts." in: Journal of virology, Vol. 85, Issue 5, pp. 2100-11, (2011) (PubMed).

Li, Hou, Wang, Chen, Shao, Yin: "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates." in: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7327-38, (2011) (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, (2005) (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, (2005) (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, (2005) (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, (2005) (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Molecular Weight: 52922

NCBI Accession: NP_001268433, NP_116274

UniProt: Q86TL0

Pathways: Autophagy