S100 Calcium Binding Protein B (S100B) antibody

Antigen: S100 Calcium Binding Protein B (S100B)

Synonyms: NEF, S100, S100beta

Clonality: Monoclonal

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659188

Additional Information

Alternative name: S100B

Gene ID: 6285

UniProt: P04271, NP_006263.1

Immunogen: This S100B monoclonal antibody is generated from mouse immunized with S100B recombinant protein.

Isotype: IgM, kappa chain

Description: Other names: Protein S100-B,S100B,

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This S100B monoclonal antibody is generated from mouse immunized with S100B recombinant protein.

Molecular Weight: 10713 DA

Comments:

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21, however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~1:5~250

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.

Storage: S100B Antibody can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Structure

Restrictions: For Research Use only

Publications

Product: Lin, Yang, Wilder et al.: "The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant melanoma." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27487-98, 2010 (PubMed).

van Dieck, Lum, Teufel et al.: "S100 proteins interact with the N-terminal domain of MDM2." in: FEBS letters, Vol. 584, Issue 15, pp. 3269-74, 2010 (PubMed).

Egberts, Momkvist, Egberts et al.: "Serum S100B and LDH are not useful in predicting the sentinel node status in melanoma patients." in: Anticancer research, Vol. 30, Issue 5, pp. 1799-805, 2010 (PubMed).

Sahoo, Tröger, Heinemann et al.: "Current inhibition of human EAG1 potassium channels by the Ca2+ binding protein S100B." in: FEBS letters, Vol. 584, Issue 18, pp. 3896-900, 2010 (PubMed).