5 lipoxygenase/ALOX5 antibody (Biotin)

Antigen: 5 lipoxygenase/ALOX5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Horse (Equine), Pig (Porcine), Dog (Canine), Rabbit

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN669653

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human 5 Lipoxygenase

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: 5-lipoxygenase, 5-LOX, 5 LO, 5LPG, ALOX 5, ALOX5, Arachidonate 5 lipoxygenase, Arachidonic acid 5 lipoxygenase, Leukotriene A4 synthase, LOG 5, LOG5, MGC163204.

Molecular Weight: 78kDa

Comments:

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein

which is expressed specifically in bone marrow-derived cells

catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8

11

14-cis-eicosatetraenoic acid

and further to the allylic epoxide 5(S)-trans-7

9-trans-11

14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed

but their full-length nature has not been determined.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only