PEDF antibody (AA 201-300) (HRP)

Catalog No. ABIN672465

The HRP-conjugated Rabbit Polyclonal anti-PEDF antibody (ABIN672465) specifically detects PEDF in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for PEDF antibody (AA 201-300) (HRP) (ABIN672465)

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEDF antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PEDF Antibody (HRP)

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse PEDF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: PEDF

Background:

Synonyms: Pedf, Sdf3, EPC-1, Pedfl, AI195227, Pigment epithelium-derived factor, Caspin, Serpin F1, Stromal cell-derived factor 3, SDF-3, Serpinf1

Background: Neurotrophic protein, induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Gene ID: 20317

UniProt: P97298