PEDF antibody (AA 19-417)

Catalog No. ABIN7428680

This Mouse Monoclonal antibody specifically detects PEDF in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for PEDF antibody (AA 19-417) (ABIN7428680)

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PEDF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C3

Product Details anti-PEDF Antibody

Binding Specificity: AA 19-417

Purpose: Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF)

Specificity: The antibody is a mouse monoclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Pigment Epithelium Derived Factor (PEDF) corresdonding to Ser19~Thr417 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: PEDF

Background: SERPINF1, EPC1, PIG35, SDF3, Serpin F1, Cell proliferation-inducing gene 35, Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin), Stromal Cell Derived Factor 3

UniProt: P97298