DARPP32 antibody (pThr75)

Catalog No. ABIN684403

This Rabbit Polyclonal antibody specifically detects DARPP32 in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for DARPP32 antibody (pThr75) (ABIN684403)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DARPP32 Antibody

Binding Specificity: pThr75

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of Thr75

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP32

Background:

Synonyms: DARPP32 phospho T75, p-DARPP32 phospho T75, PPP1R1Bphospho T75, DARPP 32, Dopamine and cAMP regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein DARPP32, FLJ20940, IPPD, Neuronal phosphoprotein DARPP 32, PPP1R1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory subunit 1B, PPR1B_HUMAN.

Background: This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

Gene ID: 84152