ARHGEF12 antibody (AA 515-615) (HRP)

Catalog No. ABIN705335

The Rabbit Polyclonal anti-ARHGEF12 antibody is suitable to detect ARHGEF12 in samples from Mouse. It has been validated for ELISA, WB, IHC (p) and IHC (fro).

Quick Overview for ARHGEF12 antibody (AA 515-615) (HRP) (ABIN705335)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ARHGEF12 Antibody (HRP)

Binding Specificity: AA 515-615

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LARG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: LARG

Background:

Synonyms: ARHGEF12, KIAA0382, leukemia-associated rho guanine nucleotide exchange factor, Leukemia-associated RhoGEF, PRO2792, Rho guanine exchange factor GEF 12, Rho guanine nucleotide exchange factor GEF 12, Rho guanine nucleotide exchange factor 12, Similar to Mouse Lsc oncogene, ARHGC_HUMAN.

Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein is observed to form myeloid/lymphoid fusion partner in acute myeloid leukemia (from EntrezGene). LARG has also been implicated in linking heterotrimeric G proteins to microtubule organisation in regulation of cell polarity.

Gene ID: 23365

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling