ARHGEF12 antibody (AA 4-254)

Catalog No. ABIN7601615

The Rabbit Polyclonal anti-ARHGEF12 antibody (ABIN7601615) specifically detects ARHGEF12 in ELISA, WB and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for ARHGEF12 antibody (AA 4-254) (ABIN7601615)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ARHGEF12 Antibody

Binding Specificity: AA 4-254

Purpose: Anti-LARG/ARHGEF12 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LARG/ARHGEF12 Antibody (ABIN7601615). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: T4-S254).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: ARHGEF12

Background:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 220 kDa

Gene ID: 23365

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling