PPM1A antibody (pSer375)

Catalog No. ABIN711761

The Rabbit Polyclonal anti-PPM1A antibody is suitable to detect PPM1A in samples from Human. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for PPM1A antibody (pSer375) (ABIN711761)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PPM1A Antibody

Binding Specificity: pSer375

Cross-Reactivity: Human

Predicted Reactivity: Dog,Cow,Sheep,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PPM1A around the phosphorylation site of Ser375

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPM1A

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: PPM1A

Background:

Synonyms: PPM1Aphospho S375, Mpp alpha, PP2C alpha, PP2C-alpha, PP2CA, PPM 1A, PPM1A, PPM1A_HUMAN, PPPM1A, Protein phosphatase 1A formerly 2C magnesium dependent alpha isoform, Protein phosphatase 1A, Protein phosphatase 1A magnesium dependent alpha, Protein phosphatase 2C alpha, Protein phosphatase 2C alpha isoform, Protein phosphatase 2C isoform alpha, Protein phosphatase IA, Protein phosphatase 2C isoform alpha, IA antibody.

Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].

Gene ID: 5494