CKMT/Creatine kinase MT antibody (Biotin)

Antigen: CKMT/Creatine kinase MT

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN717538

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from hu CKMT/Creatine kinase MT

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: Acidic type mitochondrial creatine kinase, CKMT, CKMT1, CKMT1A, CKMT1B, Creatine kinase mitochondrial 1 ubiquitous, Creatine kinase ubiquitous mitochondrial, Mia CK, Ubiquitous mitochondrial creatine kinase, UMTCK.

Molecular Weight: 47kDa

Comments:

Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier

creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes

sarcomeric MtCK and ubiquitous MtCK

encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers

in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase

this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only