F-Actin antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Sheep (Ovine), Pig (Porcine)
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Application
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Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN731498 |
| Quantity |
100 µg (1mg/1ml) |
| Price |
255.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human F-Actin
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonym: f actin, Filamentous actin.
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Molecular Weight
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42kDa
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Comments
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells .G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling in which filament length remains constant and actin monomers add at one end and dissociated at the other.
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Application Notes
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WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
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Concentration
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1mg/1ml
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Purification
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affinity purified by Protein A
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Restrictions
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For Research Use only
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Alternatives for antigen "F-Actin", type "Antibodies"
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Hosts
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Rabbit (19), Mouse (2)
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Reactivities
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Human (21), Mouse (Murine) (19), Rat (Rattus) (19), Cow (Bovine) (15), Pig (Porcine) (14), Sheep (Ovine) (14), Chicken (1), Dog (Canine) (1)
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Applications
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Flow Cytometry (FACS) (19), Immunofluorescence (IF) (17), ELISA (3), Western Blotting (WB) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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