Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) antibody

Antigen: Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12)

Synonyms: HME, MME, MGC138506, Mmel, AV378681, Mme, MMP12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN735413

Additional Information

Alternative name: MMP-12

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human MMP12 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: matrix metalloproteinase 12, MMP12, EC 3.4.24.65, HumanE, Macrophage elastase, Macrophage metaloelastase, Matrix metalloprotease 12, ME, MGC138506, MME, MMP 12.

Molecular Weight: 45kDa

Comments:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes

such as embryonic development

reproduction

and tissue remodeling

as well as in disease processes

such as arthritis

metastasis

and atherosclerosis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.MMP12 was first described in murine macrophages

later in human macrophages

and more recently in other cell types. Also known as metalloelastase

MMP12 is able to degrade elastin

entactin

laminin 1

fibronectin

type IV collagen as well as insulin B-chain and casein. MMP12 is often confused with the Serine proteinase

Leukocyte elastase (EC 3.4.21.37) because of similar nomenclature. MMP12 is structurally similar to the classical MMPs (MMP1

MMP3)

it contains a propeptide with autoinhibitory cysteine switch site

a well-conserved zinc site

hinge region and hemopexin domain. MMP12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kD

and is quickly activated to the 45 kD form

and this breaks down to cascade of active forms

ending with the common 22 kD form. Stimulated macrophages produce MMP12

it has also been found in osteosarcoma cells

synovial fibroblasts and lung fibroblasts.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Extracellular Matrix

Restrictions: For Research Use only