NR4A1 antibody (pSer351)

Catalog No. ABIN744458

The Rabbit Polyclonal anti-NR4A1 antibody is suitable to detect NR4A1 in samples from Mouse. It has been validated for ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for NR4A1 antibody (pSer351) (ABIN744458)

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NR4A1 Antibody

Binding Specificity: pSer351

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Nur77 around the phosphorylation site of Ser351

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: Nur77

Background:

Synonyms: NR4A1phospho S351, GFRP 1, GFRP, GFRP1, Growth factor inducible nuclear protein N10, Growth Factor Inducible Nuclear Protein NP10, Growth Factor Response Protein 1, Hbr1, HMR, Hormone Receptor, MGC9485, N10, N10 nuclear protein, NAK 1, NAK1, NGFIB, NP 10, NP10, NR4A1, Nuclear hormone receptor NUR/77, Nuclear Hormone Receptor TR3, Nuclear Receptor Subfamily 4 Group A Member 1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor NR4A1, ST 59, ST59, Steroid receptor TR3, TR 3, TR3, TR3 orphan receptor, Early response protein NAK1.

Background: NUR77 is a member of the steroid/thyroid hormone receptor superfamily. The gene for NUR77 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis. It has been demonstrated that mouse NUR77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. NUR77 is a phosphoprotein and its size ranges from 67 kDa to 88 kDa, depending on post-translational modifications.

Gene ID: 3164

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway