Pka R2/Pka Iialpha antibody

Catalog No. ABIN751798

This Rabbit Polyclonal antibody specifically detects Pka R2/Pka Iialpha in IF (p), IHC (p) and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Pka R2/Pka Iialpha antibody (ABIN751798)

Target: Pka R2/Pka Iialpha

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PKA R2

Isotype: IgG

Application Details

Application Notes: WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Pka R2/Pka Iialpha

Background:

Synonyms: KAP2, KAP2_HUMAN, MGC3606, PKR 2, PKR2, PRKA R2, PRKAR 2, PRKAR2, PRKAR2A, Protein kinase A RII alpha subunit, Protein kinase cAMP dependent regulatory type II alpha, cAMP dependent protein kinase regulatory subunit alpha 2, cAMP dependent protein kinase regulatory subunit RII alpha, cAMP dependent protein kinase type II alpha regulatory chain, cAMP dependent protein kinase type II alpha regulatory subunit, cAMP-dependent protein kinase type II-alpha regulatory subunit.

Background: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit

Gene ID: 5576