PRKAR2A antibody (pSer96) (HRP)

Catalog No. ABIN752677

This Rabbit Polyclonal antibody specifically detects PRKAR2A in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PRKAR2A antibody (pSer96) (HRP) (ABIN752677)

Target: PRKAR2A (Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAR2A antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKAR2A Antibody (HRP)

Binding Specificity: pSer96

Specificity: This phosphorylation site is homologous to that of Ser97 in Rat.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKAR2A

Target: PRKAR2A (Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A))

Alternative Name: PKA R2

Background:

Synonyms: PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A

Background: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Gene ID: 5576

UniProt: P13861

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection