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Antibodies
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anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) antibody (Cy5.5)
Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) antibody (Cy5.5)
| Antigen |
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| Synonyms |
PDHA, PHE1A, PDHCE1A, Pdha-1, MGC94854, MGC114215, pdha, phe1a, pdhce1a, DKFZp459C032 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Horse (Equine), Rabbit, Pig (Porcine), Chicken
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Conjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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| Catalog no. |
ABIN752853 |
| Quantity |
100 µg |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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PDHA1
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human PDHA1
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Description
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Synonym: mitochondrial, somatic form, ODPA_HUMAN, PDH, PDHA1, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial.
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Specificity
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Excitation / Emission Wavelengths: 675nm/694nm
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Comments
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The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2) and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1) dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Application Notes
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Flow-Cyt(1:50-200), IF(1:50-200)
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Purification
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Antibodies are purified by protein A and peptide affinity chromatography.
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Restrictions
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For Research Use only
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Alternatives for antigen "Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1)", type "Antibodies"
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Hosts
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Rabbit (23), Mouse (5)
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Reactivities
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Human (25), Chicken (16), Dog (Canine) (16), Mouse (Murine) (16), Pig (Porcine) (16), Rat (Rattus) (16), Cow (Bovine) (15), Horse (Equine) (14), Rabbit (14)
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Applications
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Flow Cytometry (FACS) (19), Immunofluorescence (IF) (19), Western Blotting (WB) (12), ELISA (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer293 (1)
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