Keratin Basic antibody

Catalog No. ABIN768698

The Mouse Monoclonal anti-Keratin Basic antibody is suitable to detect Keratin Basic in samples from Human. It has been validated for WB, IHC (p), IHC and IHC (fro).

Quick Overview for Keratin Basic antibody (ABIN768698)

Target: Keratin Basic

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AE3

Product Details

Specificity: This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.

Purification: Purified IgG

Immunogen: Human epidermal cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1:100), WB (1:100)

Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37 °C. Possibly, higher dilutions can be used if sections are incubated at 4 °C overnight. For use in Western blotting a dilution of 1:100 is advised.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS containing 0.1 % Sodium azide and 1 % BSA. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin