Recombinant Keratin Basic antibody

Catalog No. ABIN7862421

The Mouse Monoclonal anti-Keratin Basic antibody is suitable to detect Keratin Basic in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Keratin Basic antibody (ABIN7862421)

Target: Keratin Basic

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RKRTH-8883

Product Details

Purpose: Recombinant Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody

Specificity: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: 200ug/ml of recombinant MAb purified by Protein A/G.

Immunogen: Recombinant full-length human KRT76 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Keratin Basic

Alternative Name: Cytokeratin, Basic

Background:

KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Epithelial cells|Skin or Adenocarcinomas, Skin, A431.

Molecular Weight: 52-67kDa

Gene ID: 51350, 654392

UniProt: Q01546