MTHFD1 antibody (AA 74-123)

Catalog No. ABIN6747102

This Rabbit Polyclonal antibody specifically detects MTHFD1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig and Monkey.

Quick Overview for MTHFD1 antibody (AA 74-123) (ABIN6747102)

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTHFD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MTHFD1 Antibody

Binding Specificity: AA 74-123

Specificity: Human MTHFD1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Bovine, Horse, Guinea pig (92%) Rabbit, Pig, Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human MTHFD1 (P11586, NP_005947). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat (100%), Hamster, Elephant, Dog, Bovine, Bat, Horse, Guinea pig (92%), Rabbit, Pig, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Alternative Name: MTHFD1

Background: Name/Gene ID: MTHFD1

Synonyms: MTHFD1, C1-THF synthase, MTHFC, MTHFD

Gene ID: 4522

NCBI Accession: NP_005947

UniProt: P11586

Pathways: Methionine Biosynthetic Process