LY75/DEC-205 antibody

Catalog No. ABIN781804

Product Details anti-LY75/DEC-205 Antibody

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY75/DEC-205 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Recombinant Fc-tagged human CD205

Clone: HD30

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: CD205 / DEC-205 (LY75 Products)

Background: CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.Synonyms: C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6

Gene ID: 100526664

NCBI Accession: NP_001185688

UniProt: O60449