Goat anti-Mouse IgG3 Antibody (HRP)

Catalog No. ABIN783019

Quick Overview for Goat anti-Mouse IgG3 Antibody (HRP) (ABIN783019)

Target: IgG3

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Characteristics: Molar Ratio: Peroxidase/IgG aprox. 1.7

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.

Immunogen: Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG3

Target Type: Antibody