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OCT4 antibody (POU Class 5 Homeobox 1) (AA 232-236)

Details for Product anti-POU5F1 Antibody No. ABIN783318, Supplier: Login to see New
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Antigen
Synonyms
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, w ... show more
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06 show less
Epitope
AA 232-236
(36), (31), (31), (20), (13), (12), (9), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(305), (103), (46), (10), (10), (8), (5), (5), (5), (5), (3), (3), (3), (2), (1), (1)
Host
Rabbit
(212), (94), (31), (11)
Clonality
Polyclonal
Conjugate
This OCT4 antibody is un-conjugated
(13), (10), (10), (9), (6), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(265), (159), (56), (50), (37), (33), (30), (19), (16), (12), (8), (4), (3), (3), (3), (2), (2), (1)
Pubmed 3 references available
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Quantity 0.1 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Peptide sequence around aa.232~236 derived from OCT-4
Sequence R-K-R-T-S
Specificity This antibody detects endogenous level of total OCT-4 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity-chromatography using epitope-specific peptide
Alternative Name OCT3/4 (POU5F1 Antibody Abstract)
Background Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
Molecular Weight 45 kDa
Gene ID 5460
NCBI Accession NP_002692
UniProt Q01860
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 232-236) (ABIN783318) Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell usi...
Background publications Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, 2007 (PubMed).

Cauffman, Liebaers, Van Steirteghem et al.: "POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 12, pp. 2685-91, 2006 (PubMed).

Bruserud, Gjertsen, Foss et al.: "New strategies in the treatment of acute myelogenous leukemia (AML): in vitro culture of aml cells--the present use in experimental studies and the possible importance for future therapeutic approaches." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 1, pp. 1-11, 2001 (PubMed).

Catalog No. ABIN783318
$308.00
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