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OCT4 antibody (POU Class 5 Homeobox 1) (AA 232-236)

Details for Product anti-POU5F1 Antibody No. ABIN783319, Supplier: Login to see
Antigen
Epitope
AA 232-236
36
31
31
20
13
12
9
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
306
103
46
10
10
8
5
5
5
5
3
3
3
2
1
1
Host
Rabbit
213
94
31
11
Clonality
Polyclonal
Conjugate
This OCT4 antibody is un-conjugated
13
10
10
9
6
6
4
3
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
266
159
56
50
37
33
30
19
16
12
8
4
3
3
3
2
2
1
Supplier
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Immunogen Peptide sequence around aa.232~236 derived from OCT-4
Sequence R-K-R-T-S
Specificity This antibody detects endogenous level of total OCT-4 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity-chromatography using epitope-specific peptide
Alternative Name OCT3/4 (POU5F1 Antibody Abstract)
Background Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
Molecular Weight 45 kDa
Gene ID 5460
NCBI Accession NP_002692
UniProt Q01860
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 232-236) (ABIN783319) Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell usi...
Background publications Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, 2007 (PubMed).

Cauffman, Liebaers, Van Steirteghem et al.: "POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 12, pp. 2685-91, 2006 (PubMed).

Bruserud, Gjertsen, Foss et al.: "New strategies in the treatment of acute myelogenous leukemia (AML): in vitro culture of aml cells--the present use in experimental studies and the possible importance for future therapeutic approaches." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 1, pp. 1-11, 2001 (PubMed).