DBF4 antibody (Internal Region)

Catalog No. ABIN783975

The Goat Polyclonal anti-DBF4 antibody (ABIN783975) specifically detects DBF4 in WB and EIA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for DBF4 antibody (Internal Region) (ABIN783975)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DBF4 Antibody

Binding Specificity: Internal Region

Sequence: C-DKKRTEYLPA HEDRT

Specificity: This antibody is expected to recognize both reported isoforms (NP_038754.1 and NP_001177646.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_038754.1, NP_001177646.1.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4 / ASK

Background: DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.Synonyms: Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, Protein DBF4 homolog A, ZDBF1

Gene ID: 216

NCBI Accession: NP_001177646

Pathways: Mitotic G1-G1/S Phases, DNA Replication