GABRB3 antibody (pSer408, pSer409)

Catalog No. ABIN784601

The Rabbit Polyclonal anti-GABRB3 antibody has been validated for WB. It is suitable to detect GABRB3 in samples from Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Chicken, Non-Human Primate and Xenopus laevis.

Quick Overview for GABRB3 antibody (pSer408, pSer409) (ABIN784601)

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Chicken, Non-Human Primate, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRB3 Antibody

Binding Specificity: pSer408, pSer409

Specificity: The antibody has been directly tested for reactivity in Western blots with rat tissue. Specific for the ~53k GABAA receptor beta-3 subunit phosphorylated at Ser408/409. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, non-Human primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the di-phospho- Ser408/409 of the Rat GABAA receptor, beta-3 subunit.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABRB3

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. Phosphorylation of Serine 408 and Serine 409 within the Beta-3 subunit have been shown to be critical for the functional modulation of Beta-3 containing recombinant receptors (Brandon et al., 2000).Synonyms: GABA A receptor subunit beta-3, GABRB-3, Gamma-aminobutyric acid receptor subunit beta-3

Gene ID: 24922

NCBI Accession: NP_058761

Pathways: Sensory Perception of Sound