IL1RN antibody

Catalog No. ABIN799613

The Rabbit Polyclonal anti-IL1RN antibody is suitable to detect IL1RN in samples from Human. It has been validated for WB, IHC, ELISA, FACS and RIA.

Quick Overview for IL1RN antibody (ABIN799613)

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Radioimmunoassay (RIA)

Product Details anti-IL1RN Antibody

Specificity: Recombinant human sIL-1Ra produced in E. coli.

Purification: Purified IgG

Immunogen: Recombinant human IL1RN / IL-1RA.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA (1:500 - 1:1000), Flo, IHC, RIA, WB

Usage: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN

Background: Name/Gene ID: IL1RN
Family: IL-1

Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3

Gene ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints