PDE4B antibody

Catalog No. ABIN2728510

There is 1 publication for this product available. The Mouse Monoclonal anti-PDE4B antibody is suitable to detect PDE4B in samples from Human and Mouse. It has been validated for WB, IHC, FACS and IF.

Quick Overview for PDE4B antibody (ABIN2728510)

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1D12

Product Details anti-PDE4B Antibody

Characteristics: Homo sapiens phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant a

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human PDE4B(NP_002591) produced in HEK293T cell.

Isotype: IgG1

Application Details

Application Notes: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PDE4B antibody (ABIN2728510)

Johanns, Lai, Hsu, Jacobs, Vertommen, Van Sande, Dumont, Woods, Carling, Hue, Viollet, Foretz, Rider: "AMPK antagonizes hepatic glucagon-stimulated cyclic AMP signalling via phosphorylation-induced activation of cyclic nucleotide phosphodiesterase 4B." in: Nature communications, Vol. 7, pp. 10856, (2016) (PubMed).

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B

Background: This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 83.2 kDa

Gene ID: 5142

NCBI Accession: NM_002600

HGNC: 5142

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction