BCAT1 antibody

Catalog No. ABIN2716546

There are 2+ publications for this product available. The Mouse Monoclonal anti-BCAT1 antibody is suitable to detect BCAT1 in samples from Human, Mouse and Dog. It has been validated for WB and IHC.

Quick Overview for BCAT1 antibody (ABIN2716546)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 3F5

Product Details anti-BCAT1 Antibody

Characteristics: Homo sapiens branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:500~2000, IHC 1:150,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-BCAT1 antibody (ABIN2716546)

Cho, Jeon, Park, Park, Kang, Choi: "BCAT1 is a New MR Imaging-related Biomarker for Prognosis Prediction in IDH1-wildtype Glioblastoma Patients." in: Scientific reports, Vol. 7, Issue 1, pp. 17740, (2017) (PubMed).

Hattori, Tsunoda, Konuma, Kobayashi, Nagy, Glushka, Tayyari, McSkimming, Kannan, Tojo, Edison, Ito: "Cancer progression by reprogrammed BCAA metabolism in myeloid leukaemia." in: Nature, Vol. 545, Issue 7655, pp. 500-504, (2017) (PubMed).

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: BCAT1

Background: This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.

Molecular Weight: 42.8 kDa

Gene ID: 586

NCBI Accession: NM_005504

HGNC: 586