HSP27 antibody

Catalog No. ABIN865415

The Mouse Monoclonal anti-HSP27 antibody is suitable to detect HSP27 in samples from Human and Rat. It has been validated for WB, IHC, ELISA, IF and FACS.

Quick Overview for HSP27 antibody (ABIN865415)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5D7

Product Details anti-HSP27 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human HSP27 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF:1/200 - 1/1000, FC: 1/200 - 1/400 ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, HSPB1

Molecular Weight: 27kDa

Gene ID: 3315

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling